Accession Number : ADA018387

Title :   Freeze Preservation of Red Blood Cells with Hydroxyethyl Starch as a Cryoprotective Agent.

Descriptive Note : Annual rept.,


Personal Author(s) : Weatherbee,L.

Report Date : 01 NOV 1975

Pagination or Media Count : 32

Abstract : Pre-freeze washing increases the saline stability of red cells frozen with 14% hydroxyethyl starch (30 ml units). However, the presence of plasma in the freezing mixture does not improve the ability of the cells to survive a freeze-thaw cycle. This would allow washing before freezing to remove plasma proteins, leucocytes, etc. Large units (385-400 ml) frozen with 14% HES yield cell recoveries of 97%, saline stabilities of 82-84% and levels of supernatant hemoglobin near 550 mg%. ATP and 2-3 DPG levels drop approximately 30 and 20% respectively during freeze-thaw and potassium is lost from the cells. Storage of the thawed units at 4C for as long as 48 hours results in little change in the above parameters. Post-thaw washing causes increased damage to the cells when dilution of the freezing mixtures occurs.

Descriptors :   *Blood preservation, *Cryoprotective agents, *Erythrocytes, Freezing, Thawing, Blood cells, Stability, Hemoglobin, Adenosine phosphates, Potassium, Storage, Lysis, Saline solution

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE