Accession Number : ADA018720

Title :   An Automated DMFB Method for the Determination of Urinary Amino Nitrogen.

Descriptive Note : Final rept. Jun 74-Jul 75,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Garcia,Jesus B. , Jr. ; Bernal,Frank

Report Date : DEC 1975

Pagination or Media Count : 10

Abstract : A method using 2,4-dinitrofluorobenzene (DNFB) is described for the automatic determination of urinary amino nitrogen. Based on existing manual methods, it introduces reagents that are easily adaptable to automated techniques. A mean urinary amino nitrogen value of 77.1 mg amino N/gm creatinine was obtained for a group of healthy male subjects, compared to a range of 52 to 80 mg reported by other investigators. Direct comparison of analytical results obtained by the automated method and a manual DNFB method on several urine samples yielded a mean difference of 1.34% between the methods. (Author)

Descriptors :   *Urine, *Nitrogen, *Amines, *Chemical analysis, Automation, Biochemistry, Creatinine, Benzene compounds, Fluorine compounds, Nitrates

Subject Categories : Biochemistry
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE