Accession Number : ADA112912

Title :   Standard Operating Procedure for Mutagenicity Testing Using the Drosophila melanogaster Sex-Linked Recessive Lethal Assay.

Descriptive Note : Rept. for Jan 79-Feb 81,

Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s) : Wirtz,R A ; Powers,N R ; Fruin,J T

PDF Url : ADA112912

Report Date : Jan 1982

Pagination or Media Count : 38

Abstract : The methodology and work schedule followed at Letterman Army Institute of Research of mutagenicity testing of selected materials using the Drosophila melanogaster sex-linked recessive lethal (SLRL) assay are described. Testing procedures were designed to insure compliance with the Food and Drug Administration Good Laboratory Practices regulations. Methodology in the following areas is emphasized: procedures for stock colony maintenance and test insect handling, phenotypic descriptions of Drosophila melanogaster strains used, feeding exposure methodology for aqueous and non-water soluble materials, use of positive and negative control compounds, SLRL testing procedures, daily work schedule, data handling and statistical analysis of results. (Author)

Descriptors :   *Mutagens, *Drosophila, *Bioassay, Laboratory procedures, Insect control, Test methods, Insects, Handling

Subject Categories : Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE