Accession Number : ADA113258

Title :   Extracorporeal Perfusion without Exogenous Anticoagulation: Its Protective Role in Endotoxin Shock.

Descriptive Note : Technical rept.,


Personal Author(s) : Hinshaw,L B ; Chang,A C K ; Beller-Todd,B K ; Archer,L T ; Taylor,F B

PDF Url : ADA113258

Report Date : 19 Feb 1982

Pagination or Media Count : 37

Abstract : Our previous work demonstrated that after 90 minutes of arteriovenous perfusion without anticoagulation dogs were protected from lethal endotoxin. The present study extended that work by conducting experiments with heparinized and nonheparinized, perfused and nonperfused animals administered endotoxin. Experiments with 42 adult mongrel dogs were divided into six groups: Group A (endotoxin; no perfusion); B (perfusion; no endotoxin); C (perfusion; endotoxin initiated after 90 minutes); D (perfusion and endotoxin simultaneously); E (endotoxin plus heparin; no perfusion); F (perfusion plus heparin; endotoxin initiated after 90 minutes). Percent permanent survival ( 7 day): Group A = 14%; Group B = 100%; Group C = 86%; Group D = 29%; Group E = 29%; Group F = 57%. Survival results from Group A document the degree of lethality of the endotoxin. Observations from Group B confirm the physiological stability of the perfusion preparation. Findings from Group C document that 90 minutes preperfusion provides excellent defense against the lethality of endotoxin shock. Whole blood clotting time (WBCT) decreased in nonperfused dogs (Group A), following endotoxin while perfusion alone (Group B) and perfusion followed by endotoxin (Group C) were associated with increases in WBCT and numbers of immature neutrophils. Arterial pressure and blood glucose, total white blood cell, platelet, fibrinogen, and factor V concentrations were unaffected by perfusion but fell sharply in all groups after endotoxin.

Descriptors :   *Endotoxic shock, *Blood coagulation, Perfusion, Heparin, Protection, Dogs, Leukocytes, Therapy, Survival(General)

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE