Accession Number : ADA113370

Title :   Titration of Serum Lipoproteins with Lipoprotein Precipitants.

Descriptive Note : Interim rept. Sep 77-Oct 80,


Personal Author(s) : Clark,Dale A ; Wood,Jimmie A

PDF Url : ADA113370

Report Date : Dec 1981

Pagination or Media Count : 15

Abstract : Serum lipoproteins were titrated by sequentially increasing the concentration of precipitant in each of a series of aliquots of a serum pool or of a single serum. After standing 2 hrs at 4 C, each tube was centrifuged and the concentration of cholesterol in the supernate was measured. The precipitants tested were dextran sulfate-MnCl2 and phosphotungstate-MgCl2. As the concentration of precipitant increased, the concentration of cholesterol in the supernate decreased until the very low density lipoproteins (VLDL) and most of the low density lipoproteins (LDL), but none (or very little) of the high density lipoproteins (HDL), had precipitated. Further increases in the volume of added precipitant usually resulted in little or no decrease in the cholesterol level in the supernate until some of the HDL had precipitated. However, serum specimens differed in their response to the titrants. Titration alone sometimes gave equivocal indications of the correct HDL cholesterol level when our dextran sulfate reagent (not a commercial product) was used. Monitoring the titrations with lipoprotein electrophoresis permitted unequivocal identification of the HDL cholesterol level in most of the dextran sulfate titrations and in all our phosphotungstate titrations. These lipoprotein titrations were highly useful in establishing the optimal volume of precipitant solution and in monitoring the routine HDL cholesterol determinations. (Author)

Descriptors :   *Titration, *Lipoproteins, *Cholesterol, Phosphorus compounds, Tungstates, Magnesium compounds, Chlorides, High density, Blood serum

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE