Accession Number : ADA113489

Title :   Determination of Nucleotides by Liquid Chromatograpy with a Phosphorus Sensitive Inductively Coupled Plasma Detector.

Descriptive Note : Interim rept.,


Personal Author(s) : Heine,D R ; Denton,M B

PDF Url : ADA113489

Report Date : 14 Dec 1981

Pagination or Media Count : 29

Abstract : Quantitative analysis of 100 microliters nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HPO4 (aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus is determined with an RSD of 4.5%. High performance liquid chromatography (HPLC) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP is used as a selective detector by observing P(I) emissions at 213.6 nm. A Babington nebulizer which is used to aspirate the high percentage salt solutions with 6% efficiency is described. (Author)

Descriptors :   *Liquid chromatography, *Nucleotides, *Phosphorus compounds, *Detectors, Emission spectra, Ion exchange resins, Anions, Sensitivity, Coupling(Interaction), Quantitative analysis, Plasmas(Physics)

Subject Categories : Atomic and Molecular Physics and Spectroscopy

Distribution Statement : APPROVED FOR PUBLIC RELEASE