Accession Number : ADA115429

Title :   Effects of Pollutants on Vertebrate Cells in Vitro.

Descriptive Note : Annual rept. 1 Nov 80-31 Oct 81,

Corporate Author : FISH AND WILDLIFE SERVICE NSTL STATION MS OFFICE OF BIOLOGICAL SERVICES

Personal Author(s) : Berns,Michael W

PDF Url : ADA115429

Report Date : Jan 1981

Pagination or Media Count : 44

Abstract : Hydrazine and monomethyl hydrazine were examined with respect to their effects on vertebrate cell cultures. Comparative growth and morphological studies demonstrated that both compounds affect the cell surface. The technique of laser cell surface photobleaching of membrane probes is being developed as an assay of membrane fluidity changes. Considerable effort was devoted to perfecting this method by making numerous measurements on different cell types using two different probes. Cells treated with Hz and MMH exhibited distinct differences from the controls with respect to the % of cells showing lateral surface mobility. Furthermore a distinct difference was observed with respect to the qualitative distribution of the surface probe in control cells versus hydrazine-treated cells. Finally, correlative electron microscopy and electro-physiology demonstrated a marked effect of MMH on cell mitochondria and CA++ ion flux. (Author)

Descriptors :   *Pollutants, *Hydrazines, *Methyl hydrazines, Vertebrates, Cells(Biology), In vitro analysis, Morphology(Biology), Mitochondria, Calcium, Ions, Membranes(Biology), Fluorescence, Laser beams

Subject Categories : Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE