Accession Number : ADA115913

Title :   Viral Vaccine Immunogenicity in Relation to Host Cell-Mediated and Humoral Immune Responses.

Descriptive Note : Final rept. 14 Jun 74-13 Jun 76,

Corporate Author : NORTHWESTERN UNIV CHICAGO ILL MEDICAL SCHOOL

Personal Author(s) : Rabinowitz,Stanley G

PDF Url : ADA115913

Report Date : May 1976

Pagination or Media Count : 17

Abstract : Immune responses after immunization with a number of inactivated Venezuelan equine encephalomyelitis (VEE) virus vaccines were evaluated using an adoptive transfer system. Formalin-inactivated, TC-83 strain VEE virus vaccine was found to be immunogenic and highly effective in protecting recipients against challenge with virulent VEE virus. In contrast to immunization with live, TC-83 VEE virus vaccine, immunization with inactivated vaccine did not provide donors with the capacity to transfer adoptive immunity readily. Only when mice were immunized with inactivated VEE vaccine combined with adjuvants, particularly complete Freund's adjuvant or Bordetella pertussis, were donors capable of consistently transferring adoptive immunity. Lymphoid cell responses to immunization with inactivated VEE vaccine was next assessed by monitoring the development of both donor serum neutralizing antibody as well as adoptive neutralizing antibody responses induced by spleen cell transfer. Donors immunized intraperitoneally with formalin-inactivated VEE vaccine singly or on 3 consecutive days develop early and brisk serum neutralizing antibody responses (greater than or equal to 1:88 - 1:100) by 7 days within immunization. Recipients of spleen cells prepared from such mice are, however, incapable of eliciting a neutralizing antibody response (less than or equal to 1:10).

Descriptors :   *Immunity, *Vaccines, *Venezuelan equine encephalomyelitis virus, *Virus diseases, *Live vaccines, Response(Biology), Immunization, Spleen, Bordetella pertussis, In vitro analysis, Mice

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE