Accession Number : ADA117693
Title : Two Novel Methods for the Preparation of Erythrocyte Stroma Free from Haemoglobin Compared to the Conventional Centrifugation Technique.
Descriptive Note : Technical note,
Corporate Author : MATERIALS RESEARCH LABS ASCOT VALE (AUSTRALIA)
Personal Author(s) : Crone,H D ; Poretski,M ; Bladen,M P
PDF Url : ADA117693
Report Date : Nov 1981
Pagination or Media Count : 11
Abstract : The removal of haemoglobin from haemolysed erythrocyte stroma by washing over a membrane filter or by passage through a column of porous gel was compared to the removal achieved by washing in the centrifuge. The column method was preferable for an initial rapid clean-up, which could be followed by a further pass through the column, or by centrifugation to concentrate the suspension. Membrane filtration was useful when total recovery of a small sample of storma was required.
Descriptors : *Erythrocytes, *Membranes(Biology), *Histological techniques, Laboratory procedures, Cleaning, Filtration, Gels, Centrifuge separation, Hemolysis, Hemolysis, Hemoglobin, Blood, Solutions(General), Buffers(Chemistry), Efficiency, Timeliness
Subject Categories : Anatomy and Physiology
Test Facilities, Equipment and Methods
Distribution Statement : APPROVED FOR PUBLIC RELEASE