Accession Number : ADA117693

Title :   Two Novel Methods for the Preparation of Erythrocyte Stroma Free from Haemoglobin Compared to the Conventional Centrifugation Technique.

Descriptive Note : Technical note,


Personal Author(s) : Crone,H D ; Poretski,M ; Bladen,M P

PDF Url : ADA117693

Report Date : Nov 1981

Pagination or Media Count : 11

Abstract : The removal of haemoglobin from haemolysed erythrocyte stroma by washing over a membrane filter or by passage through a column of porous gel was compared to the removal achieved by washing in the centrifuge. The column method was preferable for an initial rapid clean-up, which could be followed by a further pass through the column, or by centrifugation to concentrate the suspension. Membrane filtration was useful when total recovery of a small sample of storma was required.

Descriptors :   *Erythrocytes, *Membranes(Biology), *Histological techniques, Laboratory procedures, Cleaning, Filtration, Gels, Centrifuge separation, Hemolysis, Hemolysis, Hemoglobin, Blood, Solutions(General), Buffers(Chemistry), Efficiency, Timeliness

Subject Categories : Anatomy and Physiology
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE