Accession Number : ADA119217

Title :   In Vitro Studies of Neurotoxic Substances: The Effect of Organophosphates and Acrylamides.

Descriptive Note : Annual technical rept. 15 Jun 81-14 Jun 82,


Personal Author(s) : Nardone,Roland M ; Spiegel,Jack ; Fedalei,Albert ; Krause,David ; Filipowski,Rene M

PDF Url : ADA119217

Report Date : 14 Aug 1982

Pagination or Media Count : 55

Abstract : The toxicity of acrylamide, n-methylacrylamide, and crotonamide as well as the organophosphates mipafox, leptophos, paraoxon, EPN, OMPA and DFP were studied in order to see whether or not in vivo-in vitro neurotoxicity correlations could be established. The in vitro systems employed were the mouse neuroblastoma cell line NIE-115 and the chick brain, either as cell aggregate cultures or organ culture. In both the neuroblastoma cell culture and chick brain cell/organ culture systems, acrylamide was the most toxic. The ranking of acrylamide, n-methylacrylamide and crotonamide paralleled the ranking reported in vivo. The end-points which showed this ranking included cell viability and neuron-specific enolase activity and aggregate formation by dissociated brain cells. The organophosphate studies emphasized their effect on neurotoxic esterase activity. A model in vitro test has been developed for the evaluation of neurotoxic esterase effects. The test is based on the hen brain assay test developed by Johnson,as a predictor of delayed neuropathy. (AUTHOR)

Descriptors :   *Toxicity, *Nerve cells, *Organophosphates, *Amides, In vitro analysis, Neuroblasts, Brain, Mice, Chickens, Cultures(Biology), Dosage, Insecticides, Esterases, Carbon oxygen lyases, Cholinesterase inhibitors, Motor neurons, In vivo analysis

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE