Accession Number : ADA119479

Title :   Calibrated Chemiluminescence Standards.

Descriptive Note : Rept. for May-Aug 82,

Corporate Author : GEORGIA UNIV ATHENS DEPT OF BIOCHEMISTRY

Personal Author(s) : Lee,John ; Matheson,Iain B C ; Losee,J R ; Zalewski,Edward F

PDF Url : ADA119479

Report Date : Aug 1982

Pagination or Media Count : 12

Abstract : The aim of this project was to provide an absolute photometric calibration for an underwater photometer in use at the Naval Oceans Systems Center in San Diego. This was made by using as a reference light standard, the chemiluminescence reaction of luminol. The quantum yield of this chemiluminescence is known. Since it was not possible to conveniently initiate the chemiluminescence reaction within the underwater photometer, it was first necessary to develop a liquid light standard which could be used for substitution. A recipe was developed for making a suspension of luminous bacteria that would emit light at a constant rate for about 30 minutes. Its photon emission rate could be easily determined using a second photometer calibrated against luminol. In the second part of the project an attempt was made to verify the published quantum yield of the luminol chemiluminescence using the new siilcon photodiode absolute photometer now available at the National Bureau of Standards. The results show satisfactory agreement with the previous measurements but the measurements are still preliminary and significant optical problems still need to be resolved. (Author)

Descriptors :   *Photometers, *Calibration, *Standards, *Chemiluminescence, Underwater, Hydrazides, Amines, Phthalic acids, Chemical derivatives, Photometry, Silicon, Photodiodes

Subject Categories : Physical Chemistry
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE