Accession Number : ADA130518

Title :   Pathogenesis of Dengue Vaccine Viruses in Mosquitoes.

Descriptive Note : Annual progress rept. no. 1, 1 Jul 79-1 Jan 80,

Corporate Author : YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE

Personal Author(s) : Beaty,Barry J ; Aitken,Thomas H G

PDF Url : ADA130518

Report Date : 01 Jan 1980

Pagination or Media Count : 23

Abstract : An in vitro assay was developed to detect transmission of Dengue-2 parent and vaccine virus strains by vector mosquitoes. After interthoracic inoculations, transmission of both viruses was detected at 7 and 14 days incubation. Rates of transmission were similar. A mosquito inoculation-headsquash-immunofluorescence technique was found to be more sensitive than Aedes albopictus cells (C6/36) for the isolation of dengue virus in the in vitro transmission assay. Flavivirus viremias in suckling mice proved relatively noninfectious for Aedes aegypti. LLC-MK2 cells were adapted to a medium containing calf serum in order to conserve fetal calf serum. Cells were maintained and grown in calf serum; fetal calf serum was used only in the overlay medium used in the plague assay test.

Descriptors :   *Dengue, *Vaccines, Culicidae, Disease vectors, Pathogenesis, In vitro analysis, Inoculation, Aedes, Infectious disease transmission, Immunoassay, Strains(Biology)

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE