Accession Number : ADA130520

Title :   Pathogenesis of Dengue Vaccine Viruses in Mosquitoes.

Descriptive Note : Annual progress rept. no. 2, 1 Jan 80-1 Jan 81,

Corporate Author : YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE

Personal Author(s) : Beaty,Barry J ; Aitken,Thomas H G

PDF Url : ADA130520

Report Date : 01 Jan 1981

Pagination or Media Count : 24

Abstract : An artificial bloodmeal feeding technique was developed which resulted in infection rates of up to 100% in mosquitoes ingesting low passage or attenuated dengue-2 viruses. The technique was used in studies to compare the relative abilities of the PR-159 parent and S-1 vaccine dengue-2 viruses to infect and to be transmitted by Aedes albopictus and Aedes aegypti mosquitoes. The S-1 vaccine candidate virus was markedly less efficient than the PR-159 parent in infection of midgut cells, dissemination from midgut cells and infection of secondary target organs, and oral transmission by the vectors. In addition the vaccine strain replicated more slowly in the vectors than the parent strain.

Descriptors :   *Dengue, *Vaccines, Culicidae, Disease vectors, Pathogenesis, In vitro analysis, Aedes, Infectious disease transmission, Gastrointestinal diseases, Oral diseases, Strains(Biology), Immunoassay

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE