Accession Number : ADA130830

Title :   Functional Role for Humoral Antibodies in Leishmaniasis in Laboratory Animal.

Descriptive Note : Final rept. 1 Feb 78-28 Feb 82, Annual rept. 1 Feb 81-Feb 82,


Personal Author(s) : Herman,Robert

PDF Url : ADA130830

Report Date : Dec 1982

Pagination or Media Count : 33

Abstract : Although stimulated and specifically and non-specifically activated macrophages are known to inhibit the replication of intracellular bacteria and some protozoan parasites in vitro, peritoneal macrophages harvested from C57B1/6J mice at various times following intravenous (i.v.) infection or superinfection with amastigotes of Leishmania donovani, supported, rather than inhibited, intracellular parasites over a nine day period. Thus, statistically significant differences, not dependent upon enhanced initial phagocytosis, were noted in amastigotes/200 cells, % of cells infected and amastigotes/infected cell in cultures of macrophages explanted 27, 65 and 118 (but not 14) days after i.v. injection of 0.5 - 1.0 10 to the 7th power amastigotes. Cycloheximide (0.5 micrograms/ml), applied to thioglycollate-stimulated macrophage cultures for four or thirteen hours prior to infection decreased, in the latter case, the number of macrophages in culture but apparently had little effect upon parasite numbers, % of cells infected or amastigotes/infected cell.

Descriptors :   *Antibodies, *Leishmaniasis, *Leishmania, *Immunity, In vitro analysis, Mice, Phagocytes, Cultures(Biology)

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE