Accession Number : ADA131010

Title :   Effects of Trypanocidal Drugs on the Function of Trypanosomes.

Descriptive Note : Annual progress rept. 1 Jan-1 Sep 78,


Personal Author(s) : Hill,George C

PDF Url : ADA131010

Report Date : 01 Sep 1978

Pagination or Media Count : 88

Abstract : The purpose of these studies has been to determine the effects of trypanocidal drugs on the function of trypanosomes. We have also been interested in determining the mode of action of trypanocidal drugs. Our approach t resolving this problem includes investigating various enzymes in host and trypanosomes, studying the effects of trypanocidal drugs on enzyme systems isolated from trypanosomes and studying the structure and transcription ability of purified kinetoplast DNA. We are interested in determining the reason for the unique selective toxicity of known trypanocidal drugs. Primary results during this past year are: (a.) established two strains of Trypanosoma brucei (T. brucei EATRO 110, and T. brucei 427) in vitro as cultured infective trypomastigotes (1); (b.) established T. rhodesiense EATRO 1895 in vitro as cultured infective trypomastigotes (2); (c.) established T. equiperdum ATCC 30023, a naturally occurring dyskinetoplastic trypanosome, in vitro as culture infective trypomastigotes; (d.) measured the inhibition of RNA and protein synthesis in T. brucei treated in vivo with berenil and suramin; and (f.) isolated purified nuclear and kinetoplast DNA from T. rhodesiense for hybridization with nuclear and mitochondrial RNA.

Descriptors :   *Trypanocides, *Trypanosoma, Response(Biology), In vitro analysis, In vivo analysis, Ribonucleic acids, Inhibition, Mitochondria, Kinetoplast, Biosynthesis, Proteins

Subject Categories : Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE