Accession Number : ADA131072

Title :   Effects of Trypanocidal Drugs on the Function of Trypanosomes.

Descriptive Note : Annual progress rept. 1 Jan-1 Sep 79,


Personal Author(s) : Hill,George C

PDF Url : ADA131072

Report Date : Sep 1979

Pagination or Media Count : 64

Abstract : During the past year, we have placed an emphasis on several aspects of our research concerned with the effects of trypanocidal drugs on the function of African trypanosomes. One of our goals this past year was to grow and maintain Trypanosomes gambiense TREU 1309 in vitro and this has been accomplished. These organisms grow as cultured-infective trypomastigotes as we have previously accomplished for several strains T. brucei and T. rhodesiense EATRO 1895 (1,2). In addition, another strain of T. brucei (LUMP 1026) has been initiated in culture. Using out in vitro culturing system, we have observed the trypanocidal effects of suramin and SHAM on the T. brucei cultured infective trypomastigotes. We have continued to study the L-a-glycerophosphate oxidase and identified an alternate electron donor, ubiquinol-1, which will be extremely important in the further characterization of this important enzyme. Finally, we have significant progress in identifying in the mitochondria of T. brucei a translation system showing sensitivity to a similar spectrum of antibiotics as that determined for eukaryotes.

Descriptors :   *Trypanocides, *Trypanosoma, Biochemistry, Cultures(Biology), In vitro analysis, Strains(Biology), Glycerols, Phosphates, Oxidoreductases, Electron donors, Electron transport, Mitochondria, Genes, Sensitivity, Antibiotics, Cytoplasm, Ribosomes, Protein metabolism, Dosage, Africa

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE