Accession Number : ADA131107

Title :   Saint Louis Encephalitis Temperature-Sensitive Mutants.

Descriptive Note : Annual rept.,

Corporate Author : MISSOURI UNIV-COLUMBIA SCHOOL OF MEDICINE

Personal Author(s) : Brawner,Thomas A

PDF Url : ADA131107

Report Date : Sep 1980

Pagination or Media Count : 24

Abstract : Saint Louis encephalitis temperature-sensitive mutants derived by induction with 5-azacytidine and 5-fluorouracil have been examined to determine complementation grouping, growth characteristics at several temperatures and virulence for mice. The results indicate that a maximum of five tentative complementation groups may be established. One mutant has been placed in groups I and II and may represent a double mutant. Examination of the ability of mutants to grow at 30 C, 40 C or 37 C indicates that some mutants are severely restricted at the nonpermissive temperature (40 C) while others are less so. Virulence studies show a separation between a-virulent mutants and those with a virulence quite similar to wild type. Mutants with a restricted growth in cell culture and a mutant with intermediate growth in cell culture were of greatly reduced virulence and behaved similarly when injected into mice.

Descriptors :   *Saint Louis Encephalitis virus, *Encephalitis, *Immunology, Strains(Biology), Mutations, Temperature, Sensitivity, Complement(Biology), Growth(General), Virulence, Mice, Live vaccines, Virus diseases, Immunity, Pathogenesis, Experimental data

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE