Accession Number : ADA131107
Title : Saint Louis Encephalitis Temperature-Sensitive Mutants.
Descriptive Note : Annual rept.,
Corporate Author : MISSOURI UNIV-COLUMBIA SCHOOL OF MEDICINE
Personal Author(s) : Brawner,Thomas A
PDF Url : ADA131107
Report Date : Sep 1980
Pagination or Media Count : 24
Abstract : Saint Louis encephalitis temperature-sensitive mutants derived by induction with 5-azacytidine and 5-fluorouracil have been examined to determine complementation grouping, growth characteristics at several temperatures and virulence for mice. The results indicate that a maximum of five tentative complementation groups may be established. One mutant has been placed in groups I and II and may represent a double mutant. Examination of the ability of mutants to grow at 30 C, 40 C or 37 C indicates that some mutants are severely restricted at the nonpermissive temperature (40 C) while others are less so. Virulence studies show a separation between a-virulent mutants and those with a virulence quite similar to wild type. Mutants with a restricted growth in cell culture and a mutant with intermediate growth in cell culture were of greatly reduced virulence and behaved similarly when injected into mice.
Descriptors : *Saint Louis Encephalitis virus, *Encephalitis, *Immunology, Strains(Biology), Mutations, Temperature, Sensitivity, Complement(Biology), Growth(General), Virulence, Mice, Live vaccines, Virus diseases, Immunity, Pathogenesis, Experimental data
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE