Accession Number : ADA131108

Title :   Saint Louis Encephalitis Temperature-Sensitive Mutants.

Descriptive Note : Final rept.,

Corporate Author : MISSOURI UNIV-COLUMBIA SCHOOL OF MEDICINE

Personal Author(s) : Brawner,Thomas A

PDF Url : ADA131108

Report Date : Jan 1981

Pagination or Media Count : 29

Abstract : Saint Louis encephalitis temperature-sensitive mutants derived by induction with 5-azacytidine and 5-fluorouracil have been examined to determine complementation grouping, RNA phenotype, growth characteristics at several temperatures, virulence for mice and the immune response to inoculation with mutant strains. The results indicate that a maximum of four complementation groups may be established. One mutant has been placed in groups I and IV; group II contains two mutants and group III contains three mutants. Examination of the ability of mutants to grow at 30 C, 40 C or 37 C indicates that some mutants are severely restricted at the nonpermissive temperature (40 C) while others are less so. Virulence studies show a separation between a-virulent mutants and those with a virulence quite similar to wild type. Mutants with a restricted growth in cell culture and a mutant with intermediate growth in cell culture were of greatly reduced virulence and behaved similarly when injected into mice. Examination of the antibody response to several mutants indicated a strong heterologous response by fourteen days post infection.

Descriptors :   *Saint Louis Encephalitis virus, *Encephalitis, *Immunology, Strains(Biology), Mutations, Temperature, Sensitivity, Complement(Biology), Ribonucleic acids, Growth(General), Virulence, Immunity, Inoculation, Live vaccines, Tissue culture cells, Antibodies, Mice, Experimental data

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE