Accession Number : ADA131624

Title :   Collection, Separation, Cryopreservation and Characterization of Peripheral Blood and Bone Marrow Stem Cells and Their Use in Treating Lethally Irradiated Dogs.

Descriptive Note : Annual rept. no. 1, 1 Jun 81-31 May 82,


Personal Author(s) : Roy,Albert J ; Melaragno,A J ; Lavigne,L T , Jr ; Gray,A D ; Dittmer,J

PDF Url : ADA131624

Report Date : 01 Oct 1982

Pagination or Media Count : 45

Abstract : Peripheral blood mononuclear cells (MNC) were obtained from the buffy coats collected during 21 platelet aphersis procedures using the Haemonetics 30 Blood Processor. The mononuclear cells in the buffy coat were divided into two equal portions. One portion was not treated. Dimethylsulfoxide (Me2SO) in McCoy's medium was added to the untreated buffy coats and to the ficoll-hypaque treated buffy coats rapidly in 1 to 2 minutes or slowly over 15 to 20 minutes. The cell suspensions were frozen in polyolefin plastic bags at 2-3 C/ min by placing the plastic bag in a -80 C freezer, or at 1 C/min by use of a graded freezing apparatus. The percentage of viable mononuclear cells recovered between the untreated buffy coat and the ficoll-hypaquue treated buffy coat depleted of contaminting red blood cells and granulocytes regardless of the rate of addition of he Me2S0-McCoy's solution and the rate of freezing. There was a significant difference in the number of viable MNC's recovered when comparing untreated buffy coat and ficoll-hypaque treated buffy coat due to a 22% loss of MNC's during ficoll-hypaque treatment.

Descriptors :   *Cryobiology, *Blood transfusion, *Blood preservation, Bone marrow, Blood platelets, Irradiation, Dogs, Freezing, Thawing, Recovery

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE