Accession Number : ADA131673

Title :   Studies on Rickettsial Diseases.

Descriptive Note : Annual progress rept. 1 Jun 72-31 May 73,


Personal Author(s) : Wisseman,Charles L , Jr

PDF Url : ADA131673

Report Date : Aug 1973

Pagination or Media Count : 47

Abstract : New methodology for the study of rickettsiae has opened the way for an enormous range of new studies of a quantitative nature. Most important among these is the routine application of modificatons of the plaquing technique refined by wike et al., the simple method for counting the absolute number of the RLB described by Silberman and Fiset and various modifications of the tissue culture technique, including the slide chamber method. These methods have permitted the recognition of extraneous agents in most yolk sac seeds of rickettsiae, plaque-purification and cloning of extraneous agents rickettsial lines, definition of the intracellular growth cycle of Rickettisia prowazeki, investigations of hose cell range of those rickettsiae and of the rickettsia-host cell interaction, methods for determining accurately the antibiotic sensitivity of rickettsiae. Reproducible, predictable in vitro systems appear to be possible. Clinical data from Burundi has confirmed our original observation that a single 100 mg dose of doxycycline will cure louse-borne typhus and has established for the first time that minocycline is equally effective. A field trail of the living attenuated E strain typhus vaccine in Bolivia confirmed its acceptability in this population.

Descriptors :   *Rickettsia, *Rickettsia prowazekii, Infectious diseases, Tissue culture, Purification, In vitro analysis, Immunity, Virulence, Genetics, Chemotherapy, Rickettsia typhi, Strains(Biology)

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE