Accession Number : ADA132459

Title :   Korean Hemorrhagic Fever.

Descriptive Note : Final rept. 1 Jan-31 Dec 80,


Personal Author(s) : Lee,Ho Wang

PDF Url : ADA132459

Report Date : 31 Mar 1981

Pagination or Media Count : 36

Abstract : The causative agent of Korean hemorrhagic fever was first isolated in 1976 from the rodent Apodemus agrarius coreae and it is registered as Hantaan virus. This report presents the results on (1) the first visualization of Hantaan virus by EM and (2) intraspecific transmission of the virus in Apodemus mice. The morphology and morphogenesis of 3 strains of Hantaan virus were examined by thin-section and negative EM of infected A549 cell-culture specimens. Virus was detected within cytoplasmic granular matrices of the infected cells. Virus particles were spherical (diameter 73 + or - 5 nm), and had on extremely electron-dense core (diameter 47 + or - 6 nm). As infection progressed, viral particles increased in number and were packed into the granular matrices as cytoplasmic crystalline arrays. Negative-contrast staining showed that the virus had an icosahedral structure and annular surface capsomers. Viruses clumped when exposed to anti-Hantaan virus serum from a convalescent patient. The morphology and morphogenesis of the virus was compatible with those of orbiviruses.

Descriptors :   *Hemorrhagic fevers, *Viruses, Korea, Electron microscopy, Morphology(Biology), Excretion, Mice, Isolation

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE