Accession Number : ADA132508

Title :   The Role of recA Protein in the Multiplicity Reactivation Pathway of Phage T4.

Descriptive Note : Master's thesis,

Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH

Personal Author(s) : McCreary,Ronald Patrick

PDF Url : ADA132508

Report Date : Jan 1983

Pagination or Media Count : 64

Abstract : The effect of two recA alleles on the multiplicity reactivation pathway of mitomycin C damaged bacteriophage T4 was studied. It was shown that both primary functions attributed to the enzyme, that of catalyzing homologous pairing and strand transfer reactions of joint molecule formation and that of a highly specific protease, are required for full function of the pathway. Additionally, the T4 gene uvsX, reported to code for a recA like protein was not found to affect the efficiency of MR. In the course of the investigation, it was noted that starving the host cells prior to phage infection appeared to induce MR. Evidence is presented that this induction occurs by a recA mediated mechanism.

Descriptors :   *Genetics, Escherichia coli, Activation, Recombination reactions, Deoxyribonucleic acids, Ultraviolet radiation, Models, Lesions, Theses

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE