Accession Number : ADA133146

Title :   Detection and Quantitation of T-2 Mycotoxin Using a Simplified Protein Synthesis Inhibition Assay.

Descriptive Note : Interim rept.,

Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s) : Thompson,W L ; Wannemacher,R W , Jr

PDF Url : ADA133146

Report Date : 18 Jul 1983

Pagination or Media Count : 26

Abstract : A simple, rapid and sensitive bioassay is described for the detection and quantitation of T-2 mycotoxin using a protein synthesis assay in cultured cells. Increased sensitivity of the cells of the mycotoxin occurs with time up to approximately 60 min. Time and dose response curves demonstrate that an average of 10 to 20 ng/ml T-2 is sufficient to cause 50% inhibition of protein synthesis in tissue culture cells. A wide range of tissue culture cells having varied type, tissue, and species sources, and growth characteristics were tested using this system. All showed approximately the same sensitivity to the mycotoxin. Using a slight modification of the procedure, suspended cultures of mitogen-stimulated lymphocytes also demonstrated an equal degree of sensitivity to the mycotoxin. By simple changing the labeled precursor, the inhibition of RNA, DNA and protein synthesis by T-2 mycotoxin can be compared. Although T-2 has little effect of RNA synthesis, DNA and protein synthesis are equally inhibited.

Descriptors :   *Phytotoxins, *Bioassay, Detection, Quantitative analysis, Protein metabolism, Inhibition, Tissue culture cells, Sensitivity, Time, Dosage, Toxicity, Lymphocytes

Subject Categories : Biochemistry
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE