Accession Number : ADA133926

Title :   Studies of Organophosphate Effects on Retinal Physiology, Cell Biology and Biochemistry.

Descriptive Note : Annual rept. 1 Apr 82-31 Mar 83,

Corporate Author : YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE

Personal Author(s) : Reid,Ted W ; Stein,Peter J

PDF Url : ADA133926

Report Date : Jul 1983

Pagination or Media Count : 13

Abstract : The overall goal of this project is to determine quantitatively and qualitatively the influence of organophosphate (OP) compounds on ocular tissues, especially the retina. Initial efforts have been to develop tissue and organ culture systems of retina and lens cells. These systems have been based on our studies with a human retinal tumor cell line (retinoblastoma-Y79) developed in our laboratories. We have successfully isolated a growth factor from the tumor cell line which is required for the growth of Y79 cells. In serum-free studies we have determined the growth requirements for the retinoblastoma cells and have shown that the growth factor, which is secreted by the cells, must bind to the cell surface in order to activate growth. Preliminary experiments have been performed on the transport of H3-diisopropylfluorophosphate (DFP) through the rabbit cornea in order to determine its rate of penetration through the cornea to the aqueous humor. Results show that it takes approximately 20 minutes for the DFP to appear in the aqueous humor and this process seems to be independent of the concentration of the OP. Thus we feel this is probably a bulk transport phenomenon. To examine the effects of OPs on retinal physiology, we have recorded electroretinograms (ERGs) from isolated, superfused retinae of the marine toad, Bufo marinus. The data indicate that superfusion with DFP differentially alters tha a and b-wave components of the ERG.

Descriptors :   *Organophosphates, Retina, Physiological effects, Cells(Biology), Biochemistry, Neoplasms, Growth(Physiology), Penetration, Transport properties, Rabbits

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE