Accession Number : ADA135634

Title :   Preparation of Hemoglobin-Containing Microcapsules.

Descriptive Note : Annual rept. 1 Oct 80-30 May 81,

Corporate Author : SRI INTERNATIONAL MENLO PARK CA

Personal Author(s) : Reyes,Z

PDF Url : ADA135634

Report Date : Jun 1981

Pagination or Media Count : 23

Abstract : Stroma-free hemoglobin (SFHb) was encapsulated with lecithin-protein-polysaccharide complexes and with chemically modified polysaccharides such as ethyl cellulose (EC) and hydroxyethyl starch acetate (HESA). In all these microcapsules, the hemoglobin (Hb) retained its ability to combine reversibly with oxygen. The microcapsules made from lecithin-gum arabic-gelatin complexes had good storage stability in a refrigerator, but because of the complexity of the encapsulation procedure, Hb was denatured when scale-up of the procedure was attempted. The microcapsules made from lecithin-gelatin-chondroitin sulfate and from lecithin-gelatin-carrageenan were very flexible, but too large ( 10 micrometers) and had irregular shapes. The HESA microcapsules were small, but too fragile. Those made from EC-gum arabic had good stability, ranged in size from 0.8 to 1.2 micrometers, had wall thickness of 0.02 to 0.03 micrometers, and had oxygen-carrying properties comparable to those of SFHb.

Descriptors :   *Hemoglobin, *Blood substitutes, *Lecithin, Erythrocytes, Models, Polymers, Lipids, Albumins, Collagen, Gelatins

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE