Accession Number : ADA135802

Title :   Evaluation Studies of the DEN-2/S-1 Vaccine.

Descriptive Note : Final rept. 1 Apr 80-3 Nov 82, Annual rept. Aug 81-Nov 82,


Personal Author(s) : Kraiselburd,E

PDF Url : ADA135802

Report Date : Nov 1982

Pagination or Media Count : 9

Abstract : The purpose of this study was to determine how much dengue 2 and dengue 4 viruses were required to infect rhesus monkeys. To this effect, groups of five dengue antibody free animals were inoculated subcutaneously with serial 10 fold dilutions of dengue 2 (PR159) and dengue 4 (H241) viruses. Virus titrations were carried out simultaneously by direct plaque assay in LLC-MK2 cells, by mosquito inoculation using both Aedes aegypti and Toxorynchites amboiensis and in the monkeys. Blood samples for serology were taken at 30 and 60 days post infection. Neutralization tests revealed that 9.5 mosquito infectious do se (50) (MID)50 of dengue 2 and 22 MID(50) of dengue 4 were required to infect 50% of the monkeys. The data suggest that in receipients challenges with wild type virus, a minimum of 100 MID(50's) should be used as the virus challenge dose.

Descriptors :   *Vaccines, *Live vaccines, *Immunity, Dengue, Immune serums, Antibodies, Response(Biology), Rhesus monkeys

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE