Accession Number : ADA136280

Title :   Electron Microscopy of Intracellular Protozoa.

Descriptive Note : Annual rept. no. 1, 1 Jan-15 Aug 79,


Personal Author(s) : Aikawa,M

PDF Url : ADA136280

Report Date : 15 Aug 1979

Pagination or Media Count : 151

Abstract : The effects of immune serum on P. falciparum-infected erythrocytes grown in vitro were investigated by electron microscopy. When immune serum was added to partially synchronized cultures of P. falciparum, parasites matured normally during the first day from ring forms to trophozoites and schizonts. In contrast, the number of new rings detected two days following reinvasion was reduced by 90%, compared with cultures containing normal serum. Clusters of merozoites were seen in cultures grown with immune serum, but rarely in cultures with normal serum. Electron microscopy showed many extracellular merozoites in the culture with immune serum. The effects of immune serum on sporozoites of P. bergjei and P. knowlesi were also studied. Sporozoites incubated in normal serum interact with macrophage and actively penetrate into the macrophages. On the other hand, sporozoites incubated in immune serum showed a distinct surface coat and were phagositized by the macrophages.

Descriptors :   *Malaria, *Electron microscopy, *Plasmodium falciparum, *Leishmania, Immune serums, ERythrocytes, In vitro analysis, Interactions, Hosts(Biology)

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE