Accession Number : ADA136618

Title :   Domestic Swine in Physiological Research. VI. Effect of Splenic Erythrocyte Sequestration on Blood Volume Measurements in Conscious Immature Pigs.

Descriptive Note : Interim rept. May-Oct 83,

Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s) : Hannon,J P ; Bossone,C A ; Rodkey,W G

PDF Url : ADA136618

Report Date : Nov 1983

Pagination or Media Count : 31

Abstract : When estimated by the dilution of 51 Cr-labelled erythrocytes under near-basal conditions, immature splenectomized pigs (N=20) had a circulating erythrocyte volume of 17.8 + or - 1.64 (S.D.) ml/kg. At an assumed body to large vessel hematocrit (BH:LH) ratio of 1.0 plasma volume was 42.8 + or - 2.69 ml/kg and blood volume 60.6 + or - 3.30 ml/kg. At an assumed BH:LH ratio of 0.9 plasma volume was 49.6 + or - 3.12 ml/kg and blood volume was 67.3 + or - 3.67 ml/kg. Sham-operated pigs (N=20) had a circulating erythrocyte volume of 16.2 + or - 1.39 ml/kg, and at a BH:LH ratio of 1.0 a plasma volume was 44.4 + or - 2.96 and a blood volume was 60.5 + or - 3.78 ml/kg; at BH:LH ratio of 0.9 plasma volume was 51.1 + or - 3.42 ml/kg and blood volume was 67.2 + or - 4.12 ml/kg. Kinetic analysis of early 51 Cr loss from the circulating blood of the sham-operated pigs indicated a splenic erythrocyte sequestration of 4.51 + or - 0.89 ml/kg and a t-1/2 of 9.67 + or - 1.93 minutes for splenic erythrocyte turnover. Epinephrine injection (0.025 mg/kg) caused rapid mobilization of splenic erythrocytes in sham-operated pigs (N=6) with a hematocrit rise from 0.27 + or - 0.01 to 0.35 + or - 0.02. Similar results were obtained in sham-operated pigs (N=9) subjected to physical restraint.

Descriptors :   *Swine, *Erythrocytes, Physiology, Blood volume, Measurement, Spleen, Labeled substances, Blood circulation

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE