Accession Number : ADA138338

Title :   Viability of Stored Rabbit Erythrocytes Carrying No C3c.

Descriptive Note : Annual progress rept. 15 May 82-28 Jan 83,

Corporate Author : MASSACHUSETTS UNIV MEDICAL SCHOOL WORCESTER

Personal Author(s) : Szymanski,I O

PDF Url : ADA138338

Report Date : Jan 1983

Pagination or Media Count : 37

Abstract : The goal of this study was to evaluate whether the third component of complement, C3, that binds onto RBC during liquid storage, contributes to the preservation injury. It was shown that rabbit RBC undergo similar changes during liquid storage in CPD plasma as human RBC. The concentration of ATP in fresh rabbit RBC was higher than in human RBC while the rate of 2,3 DPG was about two times higher in french rabbit RBC than in fresh human RBc and the rate of decline of this metabolite at 4C was similar in rabbit and human RBC. Rabbit RBC stored at 4C in CPD plasma were shown to take up C3 (the third component of complement) during storage. The viability of rabbit RBC after various periods of storage at 4C was very similar to that of human RBC, indicating that rabbit RBC developed that storage lesion at 4C in CPD plasma at about the same rate as human RBC. It appears, therefore, that the rabbit model is an easy, inexpensive way to evaluate the effectiveness of new preservation technics and the effect of other variables such as transportation, on RBC preservation.

Descriptors :   *Blood storage, *Erythrocytes, *Blood preservation, Viability, Anatomical models, Rabbits

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE