Accession Number : ADA138561

Title :   Suppression of the Immune Response by Synthetic Adjuvants.

Descriptive Note : Annual rept. 1 Aug 82-30 Jul 83,


Personal Author(s) : Johnson,A G

PDF Url : ADA138561

Report Date : 28 Feb 1984

Pagination or Media Count : 11

Abstract : Although the synthetic adjuvants muramyl di-peptide (MDP), polyadenylic-polyuridylic acid complexes (poly A.poly U) as well as bacterial lipopolysaccharides enhance the antibody response when given with antigen, each also can suppress markedly this response when given before antigen. The long term objective of this study is to determine whether each adjuvant suppressed the immune response by a common or different cell and molecular signal. Our findings during the first year were as follows: Optimal conditions were determined for suppression of PEC formation by MdP and A.poly U in Balb/c mice. Suppression was induced by a single dose of 300 micrograms MDP (iv or ip) or poly A.poly U (ip) when injected 1-2 days prior to injection of antigen (SRBC). Suppression of PFC by MDP could be transferred to syngeneic recipients by whole spleen cells, by adherent cells alone or by non-adherent cells (at a somewhat lesser order of magnitude) when removed from mice 1 day after MDP. Transfer of T cells or B cells alone (purified by panning) resulted in enhancement of PFC formation. Contrariwise, suppression PFC could not be transferred to syngeneic mice by whole spleen cells removed from Balb/c mice 1 day following pretreatment with poly A.poly U. On the other hand, serum removed from mice 90 minutes after receiving poly A.poly U was found capable of suppressing cell mediated immunity, as measured by the mixed leucocyte reaction (MLR). This effect appeared to wane by 18 hr.

Descriptors :   *Immunity, Response(Biology), Antibodies, Antigens, Peptides, Nucleotides, Lipopolysaccharides, Immunosuppression, Lymphocytes

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE