Accession Number : ADA138830

Title :   Immunochemical Identification of Newcastle Disease Virus Structural Proteins,

Corporate Author : DEFENCE RESEARCH ESTABLISHMENT SUFFIELD RALSTON (ALBERTA)

Personal Author(s) : Fulton,R E ; Shew,R

PDF Url : ADA138830

Report Date : Jan 1984

Pagination or Media Count : 27

Abstract : The immunochemical reactivity of the structural polypeptides of two strains of Newcastle Disease Virus (La Sota and B1), when reacted with hyperimmune polyclonal antisera, has been studied. Methods are described for the separation of NDV proteins by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (reduced and non-reduced), the electrophoretic transfer of separated proteins to nitrocellulose paper, and the subsequent identification of NDV antigenic determinants by a sensitive enzyme-linked immunosorbent assay. Using these methods, a number of new, minor polypeptides have been detected which hitherto have been described.

Descriptors :   *Newcastle disease virus, *Peptides, Polymers, Separation, Electrophoresis, Identification, Bioassay, Immunoassay, Canada

Subject Categories : Biochemistry
      Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE