Accession Number : ADA139247

Title :   Enzymatic Conversion of Red Cells for Transfusion.

Descriptive Note : Annual rept. 1 Mar-26 Nov 80,

Corporate Author : NEW YORK BLOOD CENTER N Y

Personal Author(s) : Goldstein,J ; Shih-Siegel,J ; Lenny,L ; Siviglia,G

PDF Url : ADA139247

Report Date : 26 Nov 1980

Pagination or Media Count : 13

Abstract : As part of our ongoing studies of the properties of the exo-alpha galactosidase from green coffee beans, we have completed comparative kinetic measurements of the removal of cell surface B antigenicity by this enzyme both in the free state and when covalently attached to soluble dextran. Typical results are presented in Table I and include treatment of B erythrocytes with either alpha galactosidase, alpha galactosidase dextran conjugate or just free enzyme in the presence of dextran. Last year we reported the isolation of an alpha N-acetylgalactosaminidase (A-zyme) free of neuraminidase activity from Aspergillus niger. Although reported to be active with blood group substances, it was unable to remove A antigenicity from red cells under our conversion conditions. We are close to completing our studies with gibbon red cells which have a B-like antigen and thus provide an experimental model that can be used to preview the fate, in vivo, of enzyme treated cells before attempting similar human studies. Under our current enzyme treatment conditions gibbon cells lose all agglutinability with human anti B antiserum within 1-2 hours.

Descriptors :   *Enzymes, *Dextran, *Erythrocytes, Blood transfusion, Conversion, Hemolysis, Adenosine phosphates, In vivo analysis

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE