Accession Number : ADA139506

Title :   Effects of Trypanocidal Drugs on the Function of Trypanosomes.

Descriptive Note : Annual rept. 1 Apr 81-30 Mar 82,


Personal Author(s) : Hill,G C

PDF Url : ADA139506

Report Date : Feb 1982

Pagination or Media Count : 62

Abstract : We have continued our research on the biochemistry of African trypanosomes. We have characterized the in vitro system for growing cultured infective trypomastigotes of African tryopanosomes and evaluated the activity of trypanocidal drugs in this system. Suramin and berenil have been shown to be extremely effective. In addition, we tested allopurinol and allopurinal ribonucleoside on T. rhodesiense cultured infective trypomastigotes. Inhibitory action was observed in vitro at 10-20 ug/ml. Efforts are now under way to analyze the acid extracted cells for any aminopurinol nucleotides by HPLC technique. Further attempts were made to isolate a mitochondrial ribosome from T. brucei. While it was possible to clearly identify 9S to 12S RNA, the presumable mitochondrial RNAs of trypanosomes, it was not possible to identify ribosomal subunits where 9S RNA was separated from 12S RNA. Given the extremely small amount of material that we have been able to obtain from mitochondrial lysates, we do not believe further pursuit of this problem is warranted at this time. Efforts have been made to identify a restriction enzyme fragment of the maxicircle of kinetoplast DNA which hybridizes with a probe which is the cloned gene for cytochrome oxidase subunit II from yeast.

Descriptors :   *Trypanocides, *Trypanosoma, Biochemistry, Africa, In vitro analysis, Cultures(Biology), Mitochondria, Ribosomes, Nucleotides

Subject Categories : Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE