Accession Number : ADA139520

Title :   Distribution and Characterization of Antigens Found in Subcellular Fractions of African Trypanosomes.

Descriptive Note : Annual rept. no. 2, 20 Aug 79-20 Aug 80,


Personal Author(s) : McLaughlin,J.

Report Date : AUG 1980

Pagination or Media Count : 65

Abstract : The aim of the project is to characterize the nature and subcellular distribution of antigens in the African trypanosome, Trypanosoma rhodesiense, and to subsequently assess their potential usefulness as vaccine material. The first annual report dealt with attempts to homogenize T. rhodesiense and the use of differential centrifugation as a means of obtaining preliminary subcellular fractions. In addition a number of potential marker enzymes, for identifying the organellar derivation of the fractions, were investigated. The present report describes the continuation of this latter aspect of the study as well as the use of sucrose, and other gradient materials, for separation of cell fractions by gradient centrifugation. Also included are initial experiments, using immunoelectrophoretic procedures, to characterize the antigenicity of the various subcellular fractions.

Descriptors :   *Trypanosoma, *Immunology, Africa, Antigens, Distribution, Immunity, Enzymes

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE