Accession Number : ADA139668

Title :   Comparative Studies on the Structure of Human Adenovirus Genomes 4, 7 and 21.

Descriptive Note : Annual progress rept. 1 Jul 79-20 Jan 80,


Personal Author(s) : Padmanabhan,R

PDF Url : ADA139668

Report Date : 01 Feb 1980

Pagination or Media Count : 32

Abstract : Adenovirus Type 7 (Greider Strain) was grown in human KB cells. The virions were purified by CsCl equilibrium centrifugation, the DNA was extracted and purified. The viral genome was analyzed by restriction endonucleases EcoRl, BamHI, SmaI, HpaI, BelI, BstEII and KpnI. The restriction endonuclease patterns of the viral DNA was compared with those of three Ad 7 subgroups published by Wadell and Varsanyi (14). Patterns we obtained by cleaving Ad 7 with SalI, HpaI, Bam Hi and SmaI were identical to those obtained by cleaving Ad 7 with SalI, HpaI, Bam Hi and SmaI were identical to those obtained with strain 1058 and strain 55142 (Ad 7 vaccine strain) reported by Wadell and Varsanyi (14). However, EcoRI pattern of Ad 7 (Greider) was different from strain 1058 and 55142 in that one site mapped at 85.4 units from the left end of the DNA is missing. DNA sequence at the inverted terminal repetition of Ad 7 DNA was analyzed in order to compare Ad 7 (Greider) with Ad 3 DNA, another member of 'weakly oncogenic' group (B). Ad 2 (add Ad 5) member of non-oncogenic group (C) and with Ad 12, member of 'highly oncogenic' group (A) of human adenoviruses. Our results indicate that the length of the inverted terminal repetition of Ad 7 (Greider) is identical to Ad 3 but different from Ad 2 (or Ad 5) and Ad 12 (or Ad 18). The nucleotide sequence homology in this unique region between Ad 7 and Ad 3 is 95%. We purified the Ad 7 DNA-protein complex to establish conditions for DNA-transfection in Human Embryonic Kidney cells in vitro.

Descriptors :   *Adenoviruses, Chromosomes, Genes, Deoxyribonucleic acids, Humans, Proteins, Isolation, Bioassay

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE