Accession Number : ADA140100

Title :   Rapid Diagnosis of Arbovirus and Arenavirus Infections by Immunofluorescence.

Descriptive Note : Annual progress rept. no. 3, 1 May 79-31 Jan 80,

Corporate Author : YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE

Personal Author(s) : Casals-Ariet,J

PDF Url : ADA140100

Report Date : Jan 1980

Pagination or Media Count : 27

Abstract : Slides with 12 small circular areas 5 mm in diameter, containing cultured cells infected with a virus, spot-slides--for use as antigen in the indirect immunofluorescence (IF) test have been prepared with the following viruses: Mayaro, dengue type 3, dengue, type 4, West Nile, Oropouche and Sicilian phlebotomus fever. Similar slides containing a mixture of cells infected with different flaviviruses, dengue types 1 and 2, Japanese encephalitis (JE), Rocio, Langat and yellow fever were also prepared; hyperimmune antisera for 20 distinct group B viruses were tested with the slides and all but 4 gave a positive reaction at dilution 1:8 or higher. It was established that bloods and sera from adult mice peripherally inoculated with Banzi virus contained high concentrations of virus. Attempts were made to identify the virus rapidly with the IF technique. Blood smears failed to show specific fluorescence; dilutions of blood inoculation to cell monolayers permitted specific detection of the virus in two days. Inoculation of cell monolayers with dilutions of a Junin virus stock followed by processing and staining for the for the IF test permitted specific detection of moderately large amounts of virus 8 and 12 hours after inoculation.

Descriptors :   *Arboviruses, *Diagnosis(Medicine), *Viruses, *Virus diseases, Serology, Identification, Immunoassay, Fluorescence, Cells(Biology), Cultures(Biology), Antigens

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE