Accession Number : ADA140101

Title :   Rapid Diagnosis of Arbovirus and Arenavirus Infections by Immunofluorescence.

Descriptive Note : Annual progress rept. no. 4, 1 Feb 80-31 Jan 81,

Corporate Author : YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE

Personal Author(s) : Casals-Ariet,J

PDF Url : ADA140101

Report Date : Jan 1981

Pagination or Media Count : 27

Abstract : Polyvalent slides bearing a mixture of five cell cultures each infected with a different group B arbovirus, for use in the indirect immunofluorescence test, have been prepared. The viruses in the slide were: Banzi, Japanese encephalitis, Langat, Rocio and yellow fever. The slides gave positive reactions when tested with hyperimmune mouse sera including 6 of 7 sera for viruses not represented in the mixture. It seems advisable, however, to prepare two different complementary sets of group B polyvalent slides if a complete or nearly complete coverage is to be achieved. Inactivation of yellow fever virus-carrying spot-slides can be accomplished by immersion in dilute betapropiolactone however, the quality of the slides may be impaired by this procedure. The immunofluorescence test with spot-slides prepared with viral isolates submitted for identification has continued to prove a time and labor saving procedure for antigenic grouping of strains; the method has been used with strains origination in Fiji, Uganda and South Africa.

Descriptors :   *Diagnosis(Medicine), *Infectious diseases, *Immunoassay, Fluorescence, Serology, Cells(Biology), Cultures(Biology), Arboviruses, Virus diseases, Japanese encephalitis viruses, Yellow fever, Histological techniques, Isolation, Antigens, Strains(Biology), Antibodies

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE