Accession Number : ADA140134

Title :   Genetics of Novel Hybrid Bacteriophage and Development of Generalized Transducing System for Salmonella typhosa.

Descriptive Note : Annual progress rept. 1 Sep 79-31 Jan 80,

Corporate Author : HAHNEMANN MEDICAL COLL AND HOSPITAL PHILADELPHIA PA

Personal Author(s) : Yamamoto,N

PDF Url : ADA140134

Report Date : Feb 1980

Pagination or Media Count : 13

Abstract : The lambda immP22 hybrids, which retain the protein coat of lambda, were found to contain various lengths of homology with Salmonella phage P22. The length of the entire lambda immP22 genome also varies from strain to strain. Density gradient centrifugation revealed that the genome of a lambda immP22dis strain is 5% larger than that of a lambda immP22 strain. Hybrids lambda immP22dis are heat unstable (at 55 C) whereas lambda immP22 are rather stable. Because the lambda immP22dis genome is tightly packed in porotein coat, lambda immP22dis particles are heat unstable. Survivors after heat treatment are often found to be deletion mutants lacking the al and 9 genes of P22. Hybrids phages between Salmonella Phage P22 and coliphage phi 80 have been isolated by using E. coli-S. typhimurium hybrids. Among those hybrid phages, phi 80immP22dis hybrids carrying both immunity related genes, immC and immI regions of P22 were isolated. Since P22 tail component gene 9 and somatic antigen conversion gene al are located between the immC and immI regions of P22, we examined whether phi 80immP22dis hybrids carry these genes. Some phi 80immP22dis hybrids carry gene al but not gene 9 whereas the remaining phi 80immP22dis hybrids carry gene 9 only. No phi 80immP22 hybrid containing both the P22 genes 9 and al were found. These observations suggest that phi 80immP22dis hybrid is formed as a consequence of multiple crossovers.

Descriptors :   *Salmonella typhosa, *Bacteriophages, Genetics, Genes, Antigens, Conversion, Salmonella typhimurium, Coliphage, Immunity, Hybridization

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE