Accession Number : ADA140144

Title :   Genetic and Physical Structures of Salmonella-coli Phage Hybrids and Development of New Generalized Transducing Hybrid Phages for E. coli.

Descriptive Note : Annual progress rept. 1 Feb 82-31 Jan 83,


Personal Author(s) : Yamamoto,N

PDF Url : ADA140144

Report Date : Jan 1983

Pagination or Media Count : 14

Abstract : The frequency of hybrid formation between Salmonella phage P22 and coli mutator phage Mu is extremely low. Therefore we had to exert enormous effort to isolate hybrids. The hybrid MuimmP22 carries the entire late genes of Mu phage and the early genes, at least the c to att region of P22 phage. MuimmP22 lysogens are sensitive to P22 infection whereas P22 lysogens are immune to MuimmP22 infection because P22 has in addition to the c region the 2nd immunity (Im) region carrying the anti-repressor (ant) gene which can inactivate the c repressor. We also isolated MuimmP22dis hybrid which carries both the c and Im regions of P22 and is dismune over MuimmP22 lysogen. Although the entire late genes of MuimmP22 hybrid class are derived from Mu phage, MuimmP22 hybrid class infects a smooth P22-sensitive host but not a rough Mu-sensitive host WR4027.

Descriptors :   *Escherichia coli, *Salmonella typhimurium, *Bacteriophages, *Hybridization, Genetics, Morphology(Biology), Bacterial extracts, Isolation, Genes, Antigens, Mutations, Recombination reactions, Lysis, Immunity, Hosts(Biology)

Subject Categories : Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE