Accession Number : ADA140147

Title :   In vitro Studies of Sandfly Fever Viruses and Their Potential Significance for Vaccine Development.

Descriptive Note : Annual rept. 1 Jan 79-1 Jan 80,


Personal Author(s) : Smith,J F

PDF Url : ADA140147

Report Date : Feb 1980

Pagination or Media Count : 69

Abstract : Several aspects of the replication of sandfly fever viruses have been examined in BHK or vero cell cultures. The results have indicated that overall similarities in the profiles of virus-specific proteins exist, but that each virus directs the synthesis of a set of proteins which is recognizably different from other viruses. Several experiments have exmained the post-translational processing of proteins specified by Karimibad and Punta Toro viruses. These studies have suggested that proteolytic cleavages occur in the processing of glycoproteins and perhaps other proteins in their conversion to mature viral proteins, probably as co-translational events since they are only demonstrable in the presence of protease inhibitors. As monitored by ferritin-tagged antibodies and electron microscopy, it will be shown that the maturation and release of these viruses does not result in appreciable modification of host cell plasma membranes with viral antigens. It has also been shown that the release of virus is not inhibited by the presence of specific anti-virus antibody. The role of the nucleus with respect to virus replication has been studied in light of the knowledge that viral antigen appears to be present within these structures and that at least one bunyavirus, Bunyamwera, fails to replicate in enucleated cells.

Descriptors :   *Sandfly fever virus infection, *Vaccines, Viruses, Infectious diseases, Antigens, Cultures(Biology), Proteins, Fevers, Arboviruses, Peptides, Glycoproteins, Morphogenesis, Growth(General), Immunology, Electrophoresis, Neutralization, Precipitation, Protein metabolism, Electron microscopy, Maturation

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE