Accession Number : ADA140162

Title :   In vitro Studies of Sandfly Fever Viruses and Their Potential Significance for Vaccine Development.

Descriptive Note : Annual rept. 1 Jan 80-1 Jan 81,


Personal Author(s) : Smith,J F

PDF Url : ADA140162

Report Date : Feb 1981

Pagination or Media Count : 59

Abstract : The structural and nonstructural polypeptides synthesized by several serologically distinct strains of sandfly fever viruses have been studied by immuno-precipitation and SDS-polyacrylamide gel electrophoresis. The resulting profiles show that a similarity exists in the number and molecular weights of the major structural and nonstructural proteins, but that most strains are distinguishable by this procedure. A similar analysis of six strains of Punta Toro virus which differ markedly in their virulence for laboratory animals has demonstrated that differences are not detectable in internal or nonstructural polypeptides, but that minor differences are seen in the surface glycoproteins. The antigen specificities of a panel of monoclonal antibodies produced against Punta Toro virus have been determined by immunoprecipitation and acrylamide gel analysis. RNA extracted from Karimabad virus-infected cells and added to rabbit reticulocyte lysates directs the synthesis of the major nonstructural protein (31K) and the nucleocapsid protein (24K), as well as several other proteins which have not been detected in infected cells.

Descriptors :   *Vaccines, *Sandfly fever virus infection, In vitro analysis, Peptides, Polymers, Serology, Molecular structure, Glycoproteins, Antibodies, Antigens, Virulence, Mice

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE