Accession Number : ADA140365

Title :   The Relationship between Erythrocyte Volume and Cell Age in Humans and Baboons.

Descriptive Note : Technical rept.,


Personal Author(s) : Thompson,C B ; Galli,R L ; Melaragno,A J ; Valeri,C R

PDF Url : ADA140365

Report Date : 30 Mar 1983

Pagination or Media Count : 21

Abstract : The relationship of red blood cell size to age during steady-state hematopoiesis has been studied using erythrocytes separated on the basis of size using counterflow centrifugation. The ratio of the age-related enzyme, erythrocyte glutamic oxaloacetic transferase (EGOT), to hemoglobin (Hb) increased progressively through the fractions, suggesting a correlation between erythrocyte volume and age. Reticulocytes, while present in all fractions, were selectively enriched in the larger subpopulations. To verify the biochemical evidence that erythrocytes decrease in volume with aging, in vivo cohort labeling of red blood cells with 59Fe was performed in baboons. A similar relationship of EGOT to Hb was observed to that in the human subpopulations. While a certain amount of erythrocyte volume heterogeneity seems to be present as a result of erythropoeisis, our data support the hypothesis that red blood cells decrease in volume as they age.

Descriptors :   *Erythrocytes, *Blood volume, *Aging(Physiology), Sizes(Dimensions), Hematopoiesis, Centrifuge separation, Flow, Transferases, Hemoglobin, In vitro analysis, Labeled substances, Radioactive isotopes, Iron, Erythropoiesis, Blood counts, Humans, Baboons

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE