Accession Number : ADA140382

Title :   Size Dependent Platelet Subpopulations: Relationship of Platelet Volume to Ultrastructure Enzymatic Activity, and Function.

Descriptive Note : Technical rept.,


Personal Author(s) : Thompson,C B ; Eaton,K A ; Princiotta,S M ; Rushin,C A ; Valeri,C R

PDF Url : ADA140382

Report Date : 10 Mar 1983

Pagination or Media Count : 37

Abstract : A method for the separation of platelets on the basis of their size has been developed using counterflow centrifugation. Platelets were separated, free of plasma proteins and other cells, into 7 subpopulations. The smallest-sized platelets, designated as Fraction 1, had a mean platelet volume (MPV) of 3.94 + or - .60 cu microns (SD). Platelet function was measured by ADP-induced aggregation, serotonin uptake, and thrombin-induced release. Progressively more rapid and more complete aggregation was observed as the platelet size increased over the 7 fractions. Serotonin uptake was 4.2 times greater in the Fraction 7 platelets than in the Fraction 1 platelets. Quantitative release of serotonin following thrombin stimulation was significantly greater in the larger-sized platelets than in the smaller-sizer platelets. The observed differences in platelet aggregation, dense body content, LDH activity, and serotonin uptake and release suggest that large platelets may be functionally more important than smaller platelets.

Descriptors :   *Blood platelets, *Blood volume, *Enzymes, Centrifuge separation, Flow, Population, Sizes(Dimensions), Fractionation, Density, Electron microscopy, Agglutination, Serotonin, Absorption, Release, Lactic dehydrogenase

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE