Accession Number : ADA140402

Title :   Platelet Size as a Determinant of Platelet Function.

Descriptive Note : Technical rept.,


Personal Author(s) : Thompson,C B ; Jakubowski,J A ; Quinn,P G ; Deykin,D ; Valeri,C R

PDF Url : ADA140402

Report Date : 10 Mar 1983

Pagination or Media Count : 26

Abstract : The relationship between mean platelet volume (MPV) and platelet function was studied in human platelet subpopulations separated on the basis of size by counterflow centrifugation. Collagen induced aggregation showed a significant negative correlation between MPV and onset of aggregation and positive correlations between the MPV and the rate and extent of aggregation. Thrombin stimulation demonstrated similar relationships between MPV and the rate and extent of aggregation. In contrast, ristocetin-induced platelet agglutination occurred at a similar rate and extent in all fractions. To quantize further the differential response of the platelets we measured the content and release of ATP and beta-thromboglobulin (Beta-TG). There was a significant correlation between MPV and both ATP and Beta-TG content and a progressive increase in the absolute release of ATP and Beta-TG associated with the increase in MPV after stimulation. However, the percent release of total ATP and Beta-TG was similar in all fractions. Data indicated that the intrinsic function of platelets of different sizes is similar, but the absolute ability of platelets to affect their environments as measured by aggregation and total release of granular content is proportional to their size.

Descriptors :   *Blood platelets, Sizes(Dimensions), Functions, Blood volume, Agglutination, Collagen, Thrombin, Adenosine phosphates, Mean

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE