Accession Number : ADA140404

Title :   The Collection, Purification, and Cryopreservation of Canine Peripheral Blood Mononuclear Cells Obtained as a By-Product of Platelet Apheresis Procedures.

Descriptive Note : Technical rept.,


Personal Author(s) : Roy,A J ; Melaragno,A J ; Gray,A D ; Dittmer,J ; Lavigne,L T

PDF Url : ADA140404

Report Date : 23 Aug 1983

Pagination or Media Count : 30

Abstract : An average of 1.69 X 10 to the 9th power white blood cells was collected from each of 11 Beagel dogs during 169 routine platelet apheresis procedures using a Haemonetics Model 30 Blood Cell Separator. Seventy-four percent of the collected white blood cells were mononuclear cells. Treatment of the mononuclear cells with ficoll-hypaque to remove the granulocytes and red blood cells resulted in a loss of 25% of the mononuclear cells. After freezing with 10% DMSO, whether as buffy coats or after ficoll-hypaque treatment, thawing and washing, about 70% of the mononuclear cells were recovered as viable cells. Recovery values of viable mononuclear cells were similar whether there was rapid addition of the cryoprotectant and freezing at 2-3 C/minute in a -80 C mechanical freezer or slow addition of the cryoprotectant and freezing at 1 C/minute in a controlled-rate freezer.

Descriptors :   *Blood preservation, *Leukocytes, Cryobiology, Dogs, Collection, Purification, Separation, Freezing, Methyl sulfoxide, Recovery, Value, Viability

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE