Accession Number : ADA140410

Title :   Microwave and Temperature Effects on the Murine Ocular Lens In Vitro.

Descriptive Note : Annual summary rept. Jun 80-Aug 81,

Corporate Author : UNIVERSITY OF WESTERN ONTARIO LONDON DEPT OF BIOCHEMISTRY

Personal Author(s) : Trevithick,J R

PDF Url : ADA140410

Report Date : Jun 1980

Pagination or Media Count : 37

Abstract : Rat ocular lenses were studied after fixation and critical point drying of the tissue by scanning electron microscopy (SEM) following exposures to elevated temperatures and/or microwave irradiation in a thermostatically controlled chamber. In this way, the temperature of the lens bathing medium was set independently of the temperature increase normally associated with application of microwave power. Irradiations were done at three final temperatures and three specific absorption rates (SAR) for two durations. These were accomplished at 915 MHz in WR975 waveguide with either pulsed (Pu) or continuous wave (CW) radiation of equal average power. The parameters of the (Pu) radiation were selected to maximize the production of thermoacoustic expansion. In addition to the types of damage noted on previous progress reports: (1) immediately after pulsed microwave irradiation, holes in cell membranes (2) after incubation, for 48 hr, globular degeneration including very large globules at high SAR's and 37 C, this work has investigated possible reciprocity between time and SAR: - although dose rate and time were varied from 5 to 20 minutes, for the same total dose a similar degree of globular degeneration (a measure of cataractous damage) was observed. More conditions and time need to be investigated to confirm this observation.

Descriptors :   *Lens(Eye), *Radiation effects, *Microwaves, Eye, Rats, Irradiation, Tissue culture, Exposure(General), Vitamin E, Temperature, Radiation absorption, Rates, Continuous waves, Pulse rate, Test methods, Electron microscopy, Chambers, Radiation dosage, Membranes(Biology), Cataracts

Subject Categories : Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE