Accession Number : ADA140420

Title :   A Microaffinity Assay for Plasma Fibronectin.

Descriptive Note : Technical rept.,


Personal Author(s) : Catsimpoolas,N ; Perilli-Palmer,B ; Griffith,A L ; Valeri,C R

PDF Url : ADA140420

Report Date : 25 Mar 1983

Pagination or Media Count : 22

Abstract : The quantitation of fibronectin in human plasma has been based exclusively on immunochemical methods to-date. An alternate method is described which utilizes gelatin-coated Sepharose 4B beads to bind the fibronectin present in plasma, and by a series of washings aided by centrifugation to remove the unbound material. Elution of the protein with 6 M urea followed by a colorimetric reaction with the Bio-Rad protein dye reagent (Coomassie Blue G-250) produces a quantitative estimation of fibronectin in plasma. Twenty-two human plasma samples from healthy volunteers were analyzed by the microaffinity assay and by a commercially available immunoturbidimetric assay kit. There was a poor correlation (r = 0.5032) between the two assays. The concentration of fibronectin in plasma was 356 + or - 87 micrograms/ml by the microaffinity assay, and 270 + or - 57 micrograms/ml by the immunoturbidimetric method.

Descriptors :   *Blood proteins, *Blood plasma, *Bioassay, *Microanalysis, Quantitative analysis, Gelatins, Coatings, Beads, Centrifuge separation, Elution, Urea, Colorimetry, Dyes, Sampling, Immunochemistry, Turbidimeters, Purification, Anticoagulants, Citrates, Phosphates, Glucose, Buffers(Chemistry)

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE