Accession Number : ADA140423

Title :   Factors Governing the Subcellular Distribution of Indium-111 in Human Platelets.

Descriptive Note : Technical rept.,

Corporate Author : BOSTON UNIV MA SCHOOL OF MEDICINE

Personal Author(s) : Costa,J L ; Rushin,C ; Vecchione,J J ; Valeri,C R

PDF Url : ADA140423

Report Date : 21 Jul 1982

Pagination or Media Count : 20

Abstract : The subcellular distribution of indium-111 (In-111), and the effect of the metabolic inhibitors rotenone and 2-deoxyglucose on its uptake, retention, and subcellular distribution, have been investigated in human platelets using techniques which permit the maintenance of dense body integrity during fractionation. As with chromium-51 (Cr-51), the In-111 label appears to be located principally in the cytosolic (soluble) fraction. Equilibrium dialysis studies suggest that only 10-20% of the In-111 is associated noncovalently with non-microsomal proteins. There appears to be a relationship between the metabolic pool of nucleotides and the uptake and retention of In-111, since incubation of platelets at 37 C with metabolic inhibitors prior to labeling with In-111 reduces the amount of label taken up when compared to platelets incubated at 22 C.

Descriptors :   *Blood platelets, *Blood chemistry, *Indium, Distribution, Metabolism, Inhibitors, Absorption, Retention(General), Radioactive isotopes, Labeled substances, Fractionation, Equilibrium(General), Dialysis, Nucleotides, Incubation

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE