Accession Number : ADA140445

Title :   The Role of Anticoagulation in the Measurement of Platelet Volumes.

Descriptive Note : Technical rept.,

Corporate Author : BOSTON UNIV MA SCHOOL OF MEDICINE

Personal Author(s) : Thompson,C G ; Diaz,D D ; Quinn,P G ; Lapins,M ; Kurtz,S R

PDF Url : ADA140445

Report Date : 10 Mar 1983

Pagination or Media Count : 27

Abstract : The effect of anticoagulation on platelet size stability was studied using blood collected in seven different anticoagulants and stored at room temperature for up to eight hours. The mean platelet volume (MPV) value was most stable in blood collected in 15% ACD and ACD/Na2EDTA. In blood collected in Na2EDTA, K3EDTA, or 11.9% ACD, there was an increase in MPV in the first two hours after which the MPV's remained stable up to eight hours. Sodium citrate and heparin proved unreliable for the measurement of platelet volume. Separate experiments demonstrated that electrolyte composition, pH, tonicity, and method of calcium chelation all influenced the stability of the MPV. Of the anticoagulants studied ACD/Na2EDTA appeared to provide the best conditions of anticoagulation for both routine clinical and research laboratory measurement of the MPV. It inhibited platelet activation but left the platelets in their normal discoid shape. Platelets could be removed from the anticoagulant and studied in functional assays for up to eight hours after blood drawing.

Descriptors :   *Blood coagulation, *Anticoagulants, Blood platelets, Blood volume, Measurement, Sizes(Dimensions), Stability, Sodium compounds, Citrates, Heparin, Calcium, Chelation, Inhibition, Activation

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE