Accession Number : ADA140458
Title : Measurement of the Distribution of Indium-111 on Human Plasma Proteins Using Chromatography and Immunoprecipitation.
Descriptive Note : Technical rept.,
Corporate Author : BOSTON UNIV MA SCHOOL OF MEDICINE
Personal Author(s) : Weiblen,B J ; Melaragno,A J ; Catsimpoolas,N ; Valeri,C R
PDF Url : ADA140458
Report Date : 13 May 1982
Pagination or Media Count : 26
Abstract : The distribution of radioactivity on plasma proteins labeled by addition of Indium-111-oxine to citrated plasma was investigated. Analysis of plasma proteins separated on Sephadex G-200 columns showed that 23-36% of the In-111 was associated with proteins with molecular weight greater than 20,000 daltons and the remaining In-111 was associated with proteins with molecular weight less than 100,000 daltons, presumably transferrin. Affinity chromatography experiments showed that less than 2% of the radioactivity was associated with albumin. Further identification of the labeled proteins and quantitation of associated radioactivity was performed by precipitating specific proteins with antibodies. These studies showed that the In-111 was distributed on transferrin (54-76%), fibrinogen (11-24%), IgM (8-20%), C3 (10-21%), and haptoglobin (3-8%). In-111 associated with fibrinogen, IgM, and haptoglobin was overestimated in some experiments due to binding of In-111 labeled C32 to the antigen-antibody precipitates.
Descriptors : *Blood plasma, *Blood proteins, Radioactivity, Labeled substances, Distribution, Indium, Radioactive isotopes, Humans, Chromatography, Quantitative analysis, Precipitation, Fibrinogen, Immunoassay
Subject Categories : Biochemistry
Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE